![]() ![]() For example I have one for 40x, 100x, 200x, and 400x. To save time you will want to make a macro of each file resolution you may encounter. (we convert to JPG later in this tutorial) Your might want to change the save type in this line to JPG. Run(“Scale Bar…”, “width=400 height=8 font=50 color=Yellow background=Black location= bold”) You will want to change the following line to reflect the look and location of you scale bar ![]() This area grabs all the images that you currently have open an starts applying scale bars You will change the distance and known values based off scale information obtained in the links above. Applies a scale bar to images taken with the microscope at current camera settings. The code is provided below, but just download the example here and use your favorite editor to make modification. Step 4 Editing macros and moving them to the macro directory: You will change the save path in this line “saveAs(“Tiff”, “C:\\test\\40x\\” title) ” Pre-create the directories you plan to use to save your photos to(imagej cannot make them). I sort of assume that since you found my page you already know how to set the scale in imagej, but if not to a look at these links. I am not going to show you how to set up the scale in imagej because other people already have good tutorials up, but you will need to know how to do this step to edit my macro code. After that you should have the macro saved) Step 2: Setting up the scale in ImageJ (You really only do this step the first time you do this process. Note: you can get away without doing the step above, but it makes finding errors much more difficult. Group your image files into folders where all have identical resolution and scale. Most of the time when you take photos on a microscope or on a tripod, the scale is the same among that group of photos.ġ. ImageJ is a useful tool for adding scale bars to images without much effort, but after extensive searching I was unable to find a script that would add scale bars to large groups of images. Part 1 – ImageJ batch adding scale bars to images: The program what will be used to batch caption and crop images. This program is used to manipulate filenames into usable captions (Use metamorphose 2 beta). ImageJ: Is the photo editor we will use to generate scale bars. ![]() The following tutorials will require the use of these software packages. In addition we can batch crop in this step.īEFORE YOU START THESE TUTORIALS MAKE BACKUPS OFF ALL OF YOUR PHOTO FOLDERS YOU PLAN TO USE. Batch adding captions or watermarks to photos based on file name. Using Metamorphose to manipulate files names into usable captions on photos. Using ImageJ to add scale bars to a large number of photos at once. I have broke it up by software packages so that you have points to jump in. You may want to know all the information that this tutorial provides or just a small portion. Although this group of post is not valuable to many individuals, it may save a handful of people hundreds of hours. ![]()
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